Adipic acid is one of the top 50 bulk chemicals and primarily used as a precursor for the synthesis of nylon, lubricants, coatings, and plastic. It is mainly produced in chemical processes from petrochemical-based aromatic compound like benzene viaa route involving the intermediacy of cyclohexane, cyclohexanol, and cyclohexanone. Because of depleting petroleum reserve, recurring crisis and the heavy environmental impact, it is necessary to develop various biotechnological production processes which would provide alternative approaches. Here we describe the engineered Escherichia coli strain harboring the synthetic foreign pathways involved in cis,cis-muconic (CCM) acid biosynthesis, which can be chemically dehydrogenated to adipic acid. The synthetic pathway consists of three E. colicodon-optimized genes encoding heterologous microbial enzymes such as 3-dehydoshikimate dehydratase, protocatechuic acid decarboxylase, and catechol 1,2-dioxygenase. The engineered E. colistrain containing these synthetic genes produced a significant amount of CCM in the liquid culture, implying that this strategy paves the way for the microbial synthetic biotechnology for the industrial production of CCM.